Video 2. In vivo time-lapse recordings revealing partial EMT of basal keratinocytes in atp1b1a morphant embryo.
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DOI: 10.7554/eLife.14277.009
In vivo time-lapse recordings of mosaic atp1b1a morphant embryo with clones of basal keratinocytes labeled with membrane-bound GFP. The Tg(Ola.Actb:Hsa.hras-egfp)-bearing progenitors of these clones had been transplanted into a non-labeled host at 6 hpf. At 48 hpf, the mosaic embryo was mounted in 1.5% low-melting agarose in E3 medium and z-stacks of clusters of GFP-positive cells were recorded every five minutes with a Zeiss laser scanning microscope (Zeiss LSM710 META) for 95 min. Maximum intensity projections were processed using ImageJ software. Stills of the videos are shown in Figure 2b. Similar results were obtained for nine recordings from 9 different individuals.