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. 2016 May 16;37(8):1101–1109. doi: 10.1038/aps.2016.15

Figure 4.

Figure 4

Comparison of the mRNA expression of CD40 and CD80 in siRNA-silenced B cells and the effects of siRNA-silenced B cells on CD28 mRNA and CD154 mRNA expression in T cells. (A) The mRNA expression of CD40 and CD80 in TACI siRNA-silenced B cells was higher than normal B lymphocytes. Moreover, BAFF significantly promoted the expression of CD40 and CD80 mRNA (*P<0.01 vs B cell group. ##P<0.01 vs B cell+BAFF group. $$P<0.01 vs BTACI- cells). (B) The mRNA expression of CD40 and CD80 in BAFF-R siRNA-silenced B cells was lower than normal B lymphocytes. BAFF did not significantly promote the expression of CD40 and CD80 mRNA (*P<0.05, **P<0.01 vs B cell group. ##P<0.01 vs B cell+BAFF group). (C) The mRNA expression of CD28 and CD154 increased in the T/TACI siRNA-silenced B cell co-culture group. Moreover, BAFF significantly promoted the expression of CD28 and CD154 mRNA (*P<0.05, **P<0.01 vs T/B cell group. #P<0.05, ##P<0.01 vs T/B cell+BAFF group. $$P<0.01 vs T/BTACI- cells). (D) The expression of CD28 and CD154 in the T/BAFF-R siRNA-silenced B cell group was lower than the control co-culture group. BAFF did not significantly promote the expression of CD28 and CD154 mRNA (*P<0.05, **P<0.01 vs T/B cell group. ##P<0.01 vs T/B cell+BAFF group. $$P<0.01 vs T/BTACI- cells). Data are shown as the mean±SD of four samples pooled from three independent experiments, each performed in technical triplicates.