Figure 4.

Probucol inhibited the activations of MAPKs in the LPS-stimulated BV2 cells. BV2 cells were treated with the indicated dose of probucol (1 or 5 μmol/L) 3 h before LPS treatment (1 μg/mL) for 6 h. (A) Probucol treatment significantly decreased the LPS-induced p38 and JNK MAPK activations. Total protein (50 μg) was subjected to 10% SDS-PAGE followed by Western blotting using anti-p-p38, anti-p38, anti-p-ERK-1/2, anti-ERK-1/2, anti-p-JNK, and anti-JNK. Quantification graphs (n=3). (B) The total cytosolic and nuclear proteins were subjected to Western blotting using anti-c-Jun. Actin and lamin B were used as internal controls. Quantification graphs (n=3).