Effect of probucol on iNOS, COX-2, IL-1β and IL-6 expression in the ischemic brain. (A) Bar graph showing the mRNA levels of iNOS, COX-2, IL-1β, and IL-6 in the ischemic brain tissues 24 h after focal cerebral ischemia as assessed with real-time PCR. These gene expression levels were normalized against GAPDH. The results are expressed as the mean±SEM (n=4). *P<0.05 and **P<0.01 vs vehicle group (Veh). (B) Western blotting for IL-1β using ischemic brain lysate (n=4). *P<0.05 and **P<0.01 vs vehicle group (Veh).