Table 1. Sequences of primers used in RT-PCR and real time PCR analysis.
| Gene | Primer | Length (bp) | Sequence (5′–3′) |
|---|---|---|---|
| RT-PCR | |||
| Mouse iNOS | sense primer | 461 | CACTTGGATCAGGAACCTGAAG |
| antisense primer | CCAGCTTCTTCAATGTGGTAGC | ||
| Mouse COX-2 | sense primer | 271 | TTCAACACACTCTATCAC |
| antisense primer | AGAAGCGTTTGCGGTACT | ||
| Mouse GAPDH | sense primer | 486 | ATGACCACAGTCCATGCCATCA |
| antisense primer | TTACTCCTTGGAGGCCATGTAG | ||
| Real-time PCR | |||
| Mouse iNOS | sense primer | 93 | TCCTGGACATTACGACCCCT |
| antisense primer | AGGCCTCCAATCTCTGCCTA | ||
| Mouse COX-2 | sense primer | 178 | CATCCCCTTCCTGCGAAGTT |
| antisense primer | CATGGGAGTTGGGCAGTCAT | ||
| Mouse IL-1β | sense primer | 196 | GGATGAGGACATGAGCACCT |
| antisense primer | TCCATTGAGGTGGAGAGCTT | ||
| Mouse IL-6 | sense primer | 191 | AGTTGCCTTCTTGGGACTGA |
| antisense primer | CAGAATTGCCATTGCACAAC | ||
| Mouse GAPDH | sense primer | 83 | CCTGGCCAAGGTCATCCAT |
| antisense primer | TCTTCTGGGTGGCAGTGATG |