Table 1.

Additive screen in styrene-producing cultures of engineered E. coli.

Entry	Culture Additive	Styrene (mM)	$\raisebox{1ex}{$f^{\prime }(t-12)$}\!\left/ \!\raisebox{-1ex}{$f^{\prime }{(t-12)}_{\mathit{control}}$}\right.$
1	None	1.49	1
2	Me-β-Cyclodextrin	1.86	1.24
4	16-BAC	0.02	<1
5	SDS	1.29	<1
6	Triton X-100	1.70	1.10
7	Tween-80	1.86	1.33
8	PTS (1)	2.70	1.81
9	TPGS-750-M (2)	2.51	1.68
10	TPGS-1000 (3)	2.64	1.78
11	SPGS-550-M (nok)	0.36[e]/0.03[f]	–
12	Vitamin E O-Ac	1.38	<1
13	PTS (1)[a]	4.07	2.95
14	TPGS-1000 (3)[a]	4.36	2.78
15	TPGS-750-M (2)[a]	4.62	2.98
16	TPGS-1000 (3)[b]	5.24	3.27
17	TPGS-750-M (2)[b]	5.52	3.28
18	TPGS-750-M (2)[c]	3.95	2.56
19	n-dodecane[d]	5.50	–

Cells were grown aerobically in 100 mL of MM1 media in sealed 500 mL Erlenmeyer flasks at 32 °C with shaking at 220 rpm. Additives were added at 3.2 mM at the point-of-induction of the styrene-producing pathway (OD₆₀₀=0.6–0.8). Styrene concentrations were determined inorganic extracts of culture aliquots by GC relative to an internal standard of 1,3,5-trimethoxybenzene using a standard curve. All data is shown as an average of three independent experiments. Detailed experimental procedures and a complete list of all surfactants tested can be found in Sections S3, S4 and Table S1.

^[a]2% wt/v,

^[b]5% wt/v,

^[c]10% wt/v,

^[d]20% wt/v,

^[e]after 24 h,

^[f]after 48 h.