Skip to main content
. Author manuscript; available in PMC: 2016 Aug 4.
Published in final edited form as: J Immunol. 2016 Apr 29;196(11):4622–4631. doi: 10.4049/jimmunol.1502452

Figure 4. Neutrophil numbers are increased in the CNS in the absence of Ccr5.

Figure 4

The total number of (A) monocytes (CD45hiCD11b+Ly6ChiLy6G−) and (E) neutrophils (CD45hiCD11b+Ly6CintLy6G+) in the brain of uninfected and LGTV-infected WT and Ccr5−/− mice on days 8 and 12 post infection were assessed by flow cytometry. Representative flow cytometry plots from day 12 post infection are shown for (B) monocytes and (F) neutrophils. Cells were gated on live singlet CD45hi cells. Neutrophils were additionally gated on CD11b+ and Ly6Cint cells. The protein levels of monocyte chemoattractants (C) Ccl2 and, (D) Ccl7, and neutrophil chemoattractants (G) Cxcl1 and (H) Cxcl2 were measured by multiplex ELISA. All data are shown as mean ± SD with 4–10 mice per time point and genotype and were pooled from two independent experiments. A Student's t-test was performed for statistical analysis with *p<0.05.