Fig. 5.

Comparative enzymatic activity of thermostable and conventional designer cellulosomes versus free enzymes. Thermostable designer cellulosomes consist of the thermostable scaffoldin that integrates the thermostable enzymes and conventional designer cellulosomes contain the wild-type enzymes. Experiments were performed on Avicel as a microcrystalline cellulose substrate, at the indicated time points and temperatures. Each reaction was performed in triplicate, and standard deviations are indicated. Pictograms denote composition of the designer cellulosomes and complementary free-enzyme cocktails. See Figs. 1 and 4 and legends for descriptions