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. 2016 Aug 4;17:547. doi: 10.1186/s12864-016-2881-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Genomic DNA is suitable for HRM-based genotyping. a Protocol timeline for sample preparation. Genomic DNA extraction, two-steps Evagreen PCR and HRM-based analysis. b Genotyping of 21 adult zebrafish obtained by an incross of glra4a ^+/− parents. The HRM curve analysis discriminates wild-type (WT), heterozygous (HT) and homozygous (HM) fish. c Sequencing results from each profile identified in (B) confirmed the genotyping and the presence of the 22 base-pair deletion in the heterozygous and homozygous population