Skip to main content
. 2015 Nov 10;2(1):e1081715. doi: 10.1080/23262133.2015.1081715

Figure 2.

Figure 2.

Rheb1 hyper-activation recapitulates DISC1 knockdown-induced dendritic developmental defects of newborn neurons in the adult hippocampus. (A) Schematic diagrams of retroviral vectors used for co-expressing GFP and sh-C1 or DsRed and sh-D1. (B–D) Dendritic length and complexity of newborn neurons at 14 d post retroviral injection (dpi) to co-express GFP and sh-C1 or sh-D1 in proliferating neural progenitors in the dentate gyrus of adult WT mice, or to express GFP alone (control) or co-express GFP and Cre (Rheb1 cOE) in conditional Rheb1-S16H overexpression mice. Shown in (B) are sample projection confocal images under different conditions. Scale bar: 20 μm. Shown in (C) is a summary of total dendritic length. Numbers associated with bar graphs indicate total numbers of GFP+ cells examined from 4 animals for each condition. Values represent mean + SEM. (*: p< 0.01; Student's t-test). Shown in (D) is a summary of Sholl analysis of dendritic complexity of newborn neurons. The same groups of neurons as in Figure 2C were examined. Values represent mean + SEM (*: p < 0.01; 2-way ANOVA).