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. 2014 Nov 17;1(1):e976026. doi: 10.4161/23262133.2014.976026

Figure 2.

Figure 2.

Np95 is not expressed in differentiated neural cells. (A–D) Representative immunofluorescence images of cells cultured under various conditions. Neuroepithelial cells from E14 mice were cultured with bFGF (10 ng/ml) for 4 days, and then cultured as described below, for the indicated number of days, either to maintain the undifferentiated condition or to induce specific differentiation. (A) Undifferentiated cells: bFGF (10 ng/ml) for 2 d. (B) Neurons: 0.5% FBS for 4 d. (C) Astrocytes: bFGF (10 ng/ml), leukemia inhibitory factor (LIF) (40 ng/ml) and bone morphogenetic protein 2 (BMP2) (40 ng/ml) for 4 d. (D) Oligodendrocytes: triiodothyronine (T3) (30 ng/ml) and thyroxin (T4) (40 ng/ml) for 7 d. After each differentiation induction period, the cells in (A-D) were stained with antibodies against Np95 (A–D) (red) and either Ki67, Nestin (A), Tuj1 (B), GFAP (C) or APC (D) (green). Np95 expression was not observed in differentiated neural cells. The insets in Merge images show Hoechst staining. White arrowheads indicate representative Np95-expressing cells. Scale bars: 20 μm.