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. 2015 Sep 21;2(1):e1057313. doi: 10.1080/23262133.2015.1057313

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© 2015 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — FGF activity in the midbrain is GSK-3 dependent, but is not regulated by β-catenin. Expression of fgf8a (A–H) and pea3 (I–P) was visualized by in situ hybridization in 24 hpf embryos treated with DMSO (A, E, I, M), SU5402 at 10µM or 6µM (B, G, J, O), BIO at 4µM (C, K), SU5402 and BIO together (D, L), when over-expressing bcat (F, N), when over-expressing bcat and treated with SU5402 (H, P). Isthmus (i). Scale bars: 100µm.