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. 2016 Aug 3;1(4):e00108-16. doi: 10.1128/mSphere.00108-16

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Copyright © 2016 Cunha et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Plaque formation and viral replication in cell culture. (A) Representative fluorescence microscopy images of FMSK^hTERT.1 cells transfected with AlHV-1 BAC DNA from different constructs at 24 and 96 h posttransfection. Nontransfected cells (mock) were used as a control. Virus spreading and cytopathic effect are indicated by the formation of plaques. Green fluorescence indicates expression of green fluorescent protein encoded by the BAC cassette. BF, bright field; F, fluorescence with a fluorescein isothiocyanate (FITC) filter. Magnification, ×10. (B) Representative images of FMSK^hTERT.1 cells infected with AlHV-1^{ΔORF73/OvHV-2-ORF8} reconstituted from FMSK^hTERT.1 (BAC⁺, BAC cassette intact) or FMSK^hTERT.1/Cre cells (BAC⁻, BAC cassette excised) at 96 h postinfection.