Supplemental Figure S2.

Expression of β2 integrins on the surface of macrophages. A: FACS analyses of cell populations present in the peritoneal lavage at day 3 after TG injection. The four populations of cells were identified from their appearance on FSC/SSC dot plots (R1 to R4). Macrophages were defined as a population of cells in the R1 region that expressed Mac-1^high, CD115⁺, and α_Dβ₂⁺ (48% ± 3% of a total cell population in the peritoneum). B: Cells in the R2 region consist of macrophages that expressed Mac-1^low/α_Dβ₂⁺ (approximately 5%) and granulocytes (mainly eosinophils) that expressed Mac-1^low/α_Dβ₂⁺ (approximately 30%). The rest of the cells (approximately 17%) are lymphocytes present in R2 and R4. B: Density of Mac-1, α_Dβ₂, and α_Xβ₂ on macrophages in the R1 region determined at different time points after TG injection. Peritoneal cells isolated from the peritoneum of WT mice were stained with mAb M1/70 (anti–Mac-1), polyclonal anti-α_Dβ_2, and mAb 3.9 (anti-α_Xβ₂) and analyzed by FACS. Data are expressed as means ± SD. n = 3 to 6 experiments. **P < 0.01. FACS, fluorescent-activated cell sorting; FSC, forward scatter; mAb, monoclonal antibody; SSC, side scatter; TG, thioglycollate; WT, wild-type.