Supplemental Figure S4.
Effect of anti-αM antibody on macrophage adhesion to Permanox. Peritoneal macrophages isolated from a 3-day inflamed peritoneum of wild-type mice were preincubated without or with 20 μg/mL of anti-αM antibody M1/70. Three hundred microliters of 5 × 105cells/mL were added to the Permanox well slides and allowed to adhere for 10, 20, and 30 minutes at 37°C. Nonadherent cells were removed, and adherent cells were labeled with Alexa Fluor 546 phalloidin and DAPI. Randomly selected fields were photographed for each condition, and adherent cells were counted with the use of ImageJ software (NIH, Bethesda, MD; http://imagej.net). Data are expressed as means ± SD. n = 10 fields with approximately 300 cells per image.