Supplemental Figure S5.

Actin-containing protrusions on MGCs formed from WT and Mac-1–deficient macrophages. A: Representative images of MGCs formed from WT and Mac-1–deficient macrophages stained with Rhodamine phalloidin for actin and DAPI for nuclei. B: The number of actin-containing protrusions was calculated from confocal images of MGCs formed from WT and Mac-1–deficient macrophages after 24 and 48 hours of fusion. The total number of actin-based protrusions was counted and divided by the total number of cells in the field. Data are expressed as means ± SD. n = 3 separate experiments. Scale bar = 100 μm. MGC, multinucleated giant cell; WT, wild-type.