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. 2016 Aug;186(8):2105–2116. doi: 10.1016/j.ajpath.2016.04.001

Figure 1.

Figure 1

Characterization of macrophage populations in the peritoneal lavage of mice at different times after TG injection. A: A peritoneum lavage was isolated 0 to 21 days after TG injection in WT and Mac-1−/− mice, and the number of macrophages was determined from differential counts. B: Representative images of cells from the peritoneal lavage isolated from WT and Mac-1−/− mice at day 18 after TG injection. Cells were stained with Wright stain. C: The number of MGCs in the peritoneum of WT and Mac-1−/− mice at day 18 after TG injection were determined as a percentage of total macrophage number. D: A number of nuclei in multinucleated macrophages were isolated from the peritoneal lavage of WT and Mac-1−/− mice at day 18 after TG injection. Data are expressed as means ± SD. n = 9 to 12 mice per time point determination (3, 6, 9, 13, and 18 days) from 3 to 5 experiments (A); n = 5 resident macrophages (day 0) from 17 mice (A); n = 2 macrophages at day 21 from 3 mice (A); n = 2 to 6 mice per 3 time points (C); n = 4 (D); n ≥ 3 nuclei/cell (inset, D). *P < 0.05. MGC, multinucleated giant cell; TG, thioglycollate, WT, wild-type.