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. 2016 Aug;186(8):2105–2116. doi: 10.1016/j.ajpath.2016.04.001

Figure 7.

Figure 7

Migration of WT and Mac-1–deficient macrophages during fusion. The speed of migration of MGCs and MNCs was determined from time-lapse imaging with the use of the ImageJ TrackMate software (NIH, Bethesda, MD; http://imagej.net). Video recording was initiated after 24 hours of stimulation with IL-4 and continued for an additional 24 hours. A: Representative still frames of WT macrophages show the route traveled by an MGC (white outline) and an MNC (yellow dashed outline). B: Migration rates of WT and Mac-1–deficient cells were determined from the distances traveled by cells. MGCs and MNCs were tracked, and the speed of their migration was calculated. Four different fields were imaged simultaneously for WT and Mac-1–deficient macrophages. Data are expressed as means ± SD. n = 5 individual MGCs and 10 MNCs and representative of 2 experiments. ***P < 0.001. MGC, multinucleated giant cell; MNC, mononucleated cell; WT, wild-type.