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. 2016 Aug;186(8):2204–2219. doi: 10.1016/j.ajpath.2016.04.005

Figure 3.

Figure 3

Expression of platelet-derived growth factor (PDGF)-D protein is inhibited in Apoe−/−/Cyp1b1+/+ mice treated with 2,3′,4,5′-tetramethoxystilbene (TMS) or by Cyp1b1 gene disruption after 28 days of angiotensin (Ang) II infusion, and expression of Pdgfd, Pdgfrb, and Itga2 in aortae of Apoe−/−/Cyp1b1+/+ mice and their down-regulation in aortae from Apoe−/−/Cyp1b1−/− mice infused with Ang II. A and B: PDGF-A to -D staining in the aorta (A), and the quantified data (B). Boxed areas in A are shown at higher magnification below. C–E: Fold-changes in Pdgfd, Pdgfrb, and Itga2 mRNA expression in aorta of Ang II–infused Apoe−/−/Cyp1b1+/+ and Apoe−/−/Cyp1b1−/− mice. mRNA expression was normalized against the reference S19 mRNA. Corresponding means ± SD ΔCT values for Apoe−/−/Cyp1b1+/+ Ang II versus Apoe−/−/Cyp1b1−/−, for reference: Pdgfd, 13 ± 0.7 versus 15.6 ± 0.4; Pdgfrb, 2.9 ± 0.05 versus 6.9 ± 0.03; and Itga2, 11.7 ± 0.1 versus 19.4 ± 0.2. Data are expressed as means ± SEM (B) or as means (C–E). n = 5 per group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (Kruskal-Wallis test followed by the Dunn multiple-comparisons test). Scale bars: 50 μm (boxed areas); 200 μm (main images, all other groups); 400 μm (main image, Apoe−/−/Cyp1b1+/+ Ang II group). Original magnification: ×4 (main image, Apoe−/−/Cyp1b1+/+ Ang II group); ×10 (main images, all other groups); ×40 (boxed areas). CT, cycle threshold value; Veh, vehicle.