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. 2015 May 6;16(8):814–831. doi: 10.1111/tra.12290

Figure 2.

TRA-12290-FIG-0002-c

RNAi screen identifies endocytic factors required for MV macropinocytic trafficking. A) Host factors required for VACV MV infection are depicted in functional clusters identified by david (dashed boxes). Blue lines between genes indicate high‐confidence (>0.9) string interactions. B) Representative images of a subset of hits from the RNAi screen, nuclei (red), infected cells (green). Bar, 50 µm. C) Comparison of the effects of a subset of VACV MV hits on SFV infection. Depletion of clathrin light chain (CLTC) and Rac1 served as positive controls for SFV and VACV MVs, respectively (comparative images displayed in inset. Scale bar, 50 µm). Results are means of three independent experiments ± SD. Black dashed line is set at 100% relative to scrambled controls. Red dashed line is set at 40%, the cutoff for definition as a hit in the screen.