Figure 7.

Effect of tumicamycin on the cell wall C . difficile 630. Cells were grown in BHIS broth supplemented with 20 μg ml⁻¹ tunicamycin to an OD of 0.85 before extracting cell wall proteins with low pH glycine and precipitating the proteins in the culture supernatant.

A. Growth curve in the presence of varying concentrations (μg/ml) of tunicamycin as indicated or the solvent DMSO.

B. Cell wall proteins (CWPs) and culture supernatant proteins (SNs) from cells grown in 20 μg ml⁻¹ tunicamycin. Molecular weights expressed in kDa.