Figure 8.

Frequency of central (CD62L⁺CCR7⁺)/effector (CD62L⁻CCR7⁻) memory CD4⁺CD44⁺ T-cells.

Notes: Mice were immunized with PS-Ag, Cub-Ag, Cub-PS-Ag, and ISA206. FACS plots in (A) are representative of the mean percentages of three mice in each group. The frequency of (B) CD4⁺CD44⁺CD62L⁺CCR7⁺ T-cells (TCM) and CD4⁺CD44⁺CD62L⁻CCR7⁻ T-cells (TEM) were measured by flow cytometry. Data are expressed as the mean ± SEM. *P<0.05 and **P<0.01 vs the PS-Ag group, while ^#P<0.05 and ^##P<0.01 vs Cub-Ag group.

Abbreviations: Ag, antigen; CCR, C-C motif chemokine receptor; CD, cluster of differentiation; Cub-Ag, mixture of cubosomes and Ag; Cub-PS-Ag, mixture of cubosome-polysaccharide nanoparticles and Ag; FACS, fluorescence-activated cell sorting; PBS, phosphate-buffered saline; PS-Ag, mixture of PS and Ag; TEM, effector memory T-cells; TCM, central memory T-cells; SEM, standard error of the mean.