Fig. 2.

Under low Cl^– conditions, amiRNA:AtSLAH1 mutants had significantly reduced expression levels of AtSLAH1 and reduced shoot Cl^– compared with null segregants. Plants were grown hydroponically for 6 weeks in BNS containing 2mM NaCl (low Cl^– conditions). (A) AtSLAH1 expression in roots of all amiRNA-AtSLAH1 mutants (amiRNA:AtSLAH1_1, 2, 3 and 4) and null segregants (nulls). (B) Shoot Cl^– accumulation of amiRNA-AtSLAH1 mutants and nulls under low Cl^– conditions. (C) Correlation between transcript level of AtSLAH1 and shoot Cl^– concentration. (D) The shoot NO₃ ^–/Cl^– ratio in all amiRNA:AtSLAH1 mutant and null segregant lines grown under low Cl^– conditions. Results are mean+SEM (n>8), except (C), which is ±SEM. Statistical differences determined by one-way ANOVA and Tukey’s test (P≤0.005); a and b represent statistically significant differences between data groups.