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. 2016 Jun 23;67(15):4495–4505. doi: 10.1093/jxb/erw237

Fig. 4.

Fig. 4.

Stelar cell type-specific overexpression of AtSLAH1 in E2586 significantly increased shoot Cl– accumulation and reduced shoot biomass under high Cl– conditions. (A) Correlation between shoot Cl– accumulation and relative expression of AtSLAH1 in GAL4:AtSLAH1 overexpression lines under high Cl– (75mM NaCl) supply. Open circles and solid line: GAL4:AtSLAH1_1; open triangle and dash line: GAL4:AtSLAH1_2. Plants were grown hydroponically in BNS for 6 weeks and then exposed to BNS containing 75mM NaCl (high Cl– conditions) for 7 days. Correlation between shoot Cl– concentration and the abundance of AtSLAH1 in GAL4:AtSLAH1_1 (R 2=0.5805, P ≤ 0.005, significant deviation from zero), GAL4:AtSLAH1_2 (R 2=0.5395, P ≤ 0.005, significant deviation from zero). (B) Whole shoot biomass (fresh weight) as measured after high Cl– treatment. Results are mean+SEM (n>6). Statistical differences determined by one-way ANOVA and Tukey’s test (P≤0.05); a and b represent statistically significant differences between data groups.