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. 2016 Jun 27;67(15):4647–4658. doi: 10.1093/jxb/erw240

Fig. 4.

Fig. 4.

BjMYB1 binds to the Wbl-4 and W-box element in vitro. (A) Nucleotide sequences of the probes with central nucleotides in bold and underlined. W4 contains the Wbl-4 element. W4-d1 is a mutant of W4 with the core sequence TGAC in the Wbl-4 element deleted (----). W4-d2 is a mutant of W4 with the GTGACT changed into a typical W-box element motif TTGACC. W4-d3 is another mutant of W4 with the GTGACT changed into the AC element motif ACCTACCA. PAL2Pro is the promoter fragment of the bean PAL2 gene containing the AC element ACCTACC. (B) EMSA for the DNA-binding activity of the His-BjMYB1 fusion protein with W4, W4-d1, W4-d2, W4-d3, and PAL2Pro probes. An equal amount of BjMYB1 protein or hot probe (biotin-labelled) was used in all lanes. There was 100 times the amount of cold probes (without the biotin label) than hot probes for competitive binding. The bound and free hot probes are indicated by arrows on the left.