Fig. 5.

BjMYB1 activates BjC-P by binding to the Wbl-4 element in vivo. (A) Schematic diagram of BjC-P, GUS-expressing constructs P16, P53, and the BjMYB1-expressing construct pBjMYB1. Grey boxes represent BjC-P and its deletion derivatives. The numbers above the boxes indicate the nucleotide positions relative to the BjCHI1 transcription start site. Blue boxes represent the GUS gene containing an intron indicated by the texture area. The small white box in P53 indicates deletion of the core sequence TGAC in Wbl-4. (B) GUS histochemical staining of a tobacco leave infiltrated or co-infiltrated with the constructs as indicated. The dashed circles indicate the infiltration areas. (C) Quantization of GUS activity of P16 and P53 in transiently transformed tobacco leaves. N. benthamiana leaves were co-infiltrated with P16 or P53 and pBjMYB1 as well as a CaMV 35S::LUCint construct (pBI121-LUCint). Each of the plasmids was adjusted into equal density for the infiltrations. The GUS activity was normalized with the LUC activity. Columns show the ratio of GUS activity induced by pBjMYB1 to that of no induction. Values represent means ± SD from three replicates. The asterisks indicate significant difference (one-tail t-test, compared with control, P < 0.01).