Fig. 3.

Naa10 is required for local auxin accumulation in the early embryonic stage during embryogenesis. (A–D) and (A’–D’) wild-type embryos; (E–G) and (E’–G’) naa10-1 ^–/– embryos. (A–G) GFP signal; (A’–G’) merged images. DR5 was expressed in the root pole of the wild-type globular stage (A, A’) and heart stage (B, B’) embryos. DR5 was also expressed in wild-type cotyledon tips (C, C’) and in the vasculature of mature wild-type embryos (D, D’). DR5 was expressed throughout the arrested globular embryo as well as the suspensor in naa10-1 ^–/– embryos (E–G). (B) and (E), (C) and (F), and (D) and (G) are embryos at the same time after fertilization, respectively. The number of embryos examined was 18, 13, 15, 10, 28, 27 and 22 for (A) to (G), respectively. Scale bars =50 μm.