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. 2016 Apr 18;11(5):e1176660. doi: 10.1080/15592324.2016.1176660

Figure 4.

Figure 4.

Adventitious rooting in Arabidopsis lTCLs cultured in the presence of IBA (10 µM) and Kin (0.1 µM), genes involved, and IAA localization and transport. (A-B) Expanded cortical cells and superimposed layers of stem endodermis derivative cells organizing meristematic cell clusters (A, arrow), and meristemoids (B, arrow). (C-D) Not-yet-protruded ARP (C), and elongated AR (D) originated by root meristemoids. (E) QC definition (asterisks) in an ARP. (F) ARP showing the appearance of the expression of the pAGL42 QC-marker (green fluorescence) in the QC cells (pAGL42::GFP line). (G) WOX5 expression (green fluorescence) in a meristemoid (pWOX5::GFP line). (H-I) Protruding ARP (H) and elongated AR (I) showing SCR expression (green fluorescence) in the QC, endodermis/cortical initial cells, their derivatives, and forming endodermis (I) (pSCR::GFP line). (J-K) Initial AR-stages (J) and late stages, i.e., protruding ARPs (K), in lTCLs from the shr-1 (J) and scr-1 (K) mutants. The arrow in (J) shows a meristematic cell cluster originated from the innermost cortical layer which initiates the AR-process in the place of the lacking endodermis. (L) Meristemoid showing IAA presence detected by DR5::GUS expression (DR5::GUS line). (M) AR with LAX3 expression localized in the vasculature and procambium, but excluded by the apex (LAX3:: GUS line). (N-O) AUX1::GUS expression in the meristematic cell clusters (N) and in early-staged ARPs (O) (AUX1::GUS line). (P) Elongated, but not yet protruded, ARPs showing a PIN1 continuous signal from the forming vasculature to the tip (PIN1::GUS line). A-E (Columbia ecotype) and J-K (shr-1 and scr-1) histological longitudinal radial sections stained with toluidine blue. Insets in the fluorescence pictures (F-I) show corresponding bright-field images. All transgenic lines are in the Columbia background, except for pSCR::GFP, in the Wassilewskija background. Wassilewskija is the wild type of homozygous scr-1 and shr-1 null mutants. 22 days of culture under continuous darkness. Bars = 20 μm (E,N), 40 μm (A, H-L and insets in G-I), 60 μm (B-C, F-G, M, O-P and inset in F), 100 μm (D).