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. 2016 Sep;28(9):1380–1388. doi: 10.1016/j.cellsig.2016.06.015

Fig. 4.

Fig. 4

CRISPR cell line generation. A) Genomic region of RPIA Exon 1 on chromosome 2p11.2, showing the exon (green box), sgRNAs (fwd/rev) and genomic primers (E1 fwd/rev) used in B-D. Red arrows show the predicted nicks. B) Genomic amplification of control (CR-WT) and CRISPR-RPIA clones (CR1–3) on a 2% agarose gel. C) Sequencing reaction results of CR-WT and CR clones by Sanger sequencing. Red arrows indicate start of mixed base pair reads. D) Sequence alignment of CR-WT and CR clones sequencing reactions using ClustalW.