Fig. 5.

LC3-II levels and LC3 puncta are increased in CRISPR cell lines. A) Immunoblot of LC3-processing in control cells (CR-WT), CR-1 and CR-2, treated with 10 nM bafilomycin A (baf) for 2 h. B) Densitometry analysis of relative LC-II/Actin levels using Fiji. Data show the mean ± SD, n = 5. *, p < 0.05. C) Endogenous LC3 puncta in fixed CR-WT, CR-1 and CR-2 that were treated with 10 nM baf for 2 h. Images were acquired on an Opera LX microscope, scale bar = 10 μm. D) Quantification of C) using Columbus image analysis software. 10,000–28,000 cells per condition were analyzed in 3 independent experiments. ***, p < 0.001; n.s. = non-significant.