TABLE 1.
Sequences of various primers used in this study. F – forward and R – reverse, RT – reverse transcription.
| Designation | Sequence | Used in |
|---|---|---|
| sh-1 | CCGGCGGGTACACAAATGGAGTGAACTCGAGTTCACTCCATTTGTGTACCCGTTTTTG | Knockdown of RPIA |
| sh-2 | CCGGGCTGATGAAGTAGATGCTGATCTCGAGATCAGCATCTACTTCATCAGCTTTTTG | Knockdown of RPIA |
| sh-3 | CCGGGAATTGGAAGTGGTTCTACAACTCGAGTTGTAGAACCACTTCCAATTCTTTTTG | Knockdown of RPIA |
| sh-4 | CCGGGAAGTGAATACAGCTATCAAACTCGAGTTTGATAGCTGTATTCACTTCTTTTTG | Knockdown of RPIA, pMOWS cloning |
| RT-RPIA F | GGCGGTGCTGGCAACACAAG | RT-PCR |
| RT-RPIA R | TGGCGGGCCTGGAAGGAAGT | RT-PCR |
| GAPDH F | GAAATCCCATCACCATCTTCCAGG | RT-PCR |
| GAPDH R | GAGCCCCAGCCTTCTCCATG | RT-PCR |
| sgRNA-R | CACCGGCCGCACGTGGGAACCCGGG | CRISPR reverse strand nicking |
| sgRNA-F | CACCGTGCCGGGGCGTGCACAGTCT | CRISPR forward strand nicking |
| CRISPR gen F | GCGAATCCAGATAGGGGTTCCTCGAAGC | CRISPR - genomic PCR |
| CRISPR gen R | GCAAGCTTAGCAGGGAAGAGGGGTCTAA | CRISPR - genomic PCR |