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. 2015 Mar 18;15(16):2842–2850. doi: 10.1002/pmic.201400440

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© 2015 The Authors. Proteomics published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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ESI‐MS gas‐phase HDX of β‐lactoglobulin. ESI‐MS spectra showing the degree of deuterium incorporation experienced by β‐lactoglobulin in the absence of TFE (0TFE) and with 15% TFE (15TFE) added for (A) the 7+ ions; (B) the 8+ ions and (C) the 9+ ions. (D) The mean deuterium incorporation of the 7+, 8+ and 9+ ions in the absence of TFE and with 15% TFE added; (E) the range of deuterium incorporation into the 7+, 8+ and 9+ ions estimated by identifying the peak width at 50% peak height. All bar charts represent the mean values of three replicates; error bars are the standard deviation of the mean.