Figure 7.

Inhibition of E‐selectin expression by p38 and JNK MAPK pathway inhibitors, Inhibitors were added 30–60 min prior to 5 h stimulation with 10⁸ cfu ml⁻¹ WT and lpxA‐ bacteria or 10 ng ml⁻¹ purified LPS. E‐selectin surface expression was detected by FACS analysis, as described in the Experimental procedures section.

(A) p38 inhibition, (B) JNK inhibition, (C) p38 and JNK combined inhibition. Results are expressed as MFI E‐selectin expression ± SEM percentage inhibition of the MFI of E‐selectin expression. n = 4. (D) Table expressing percentage inhibition in E‐selectin expression induced by WT, lpxA‐ and LPS by the three inhibitors.