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. 2016 Aug 4;11(8):e0160018. doi: 10.1371/journal.pone.0160018

Fig 9. Colloidal blue stain of SDS-PAGE gel, and liquid chromatography/mass spectrometry (LC/MS) analysis.

Fig 9

D11S-1 and SCC1398 were grown in TSBYE, TSBYE + 50% human serum, or TSBYE + 50% horse serum for 23 h using polystyrene tubes. Approximately the same numbers of cells were lysed, separated using a 4–15% gradient polyacrylamide gel, and stained. Four extra bands were associated with samples prepared using human serum: ~280 kDa, ~260 kDa, ~65 kDa and ~25 kDa (shown by ‘↑’). However, only the ~25 kDa band showed 3- to 10-fold stronger signal in the sample from D11S-1/human serum, compared to the samples from either SCC1398/human serum, D11S-1/horse serum or D11S-1/TSBYE (shown by square brackets ‘[‘). The ~25 kDa bands were excised and analyzed using a LC/MS system, and partial data were shown in Table 5.