Table 3. Top 20 most up-regulated genes by human serum in the high-responder strain D11S-1.
| Genes* | Accessory | P-cluster*** | Ratio | |
|---|---|---|---|---|
| Genes** | Horse serum/TSBYE | Human serum/TSBYE | ||
| Arginine-binding periplasmic protein 1 (artI) | - | 00709 | 3.2 | 7.0 |
| Arginine transporter permease subunit (artM) | - | 00731 | 3.9 | 6.9 |
| Arginine transporter permease subunit (artQ) | - | 01364 | 4.0 | 6.8 |
| Transposase | + | 02169 | 7.5 | 6.6 |
| Hypothetical protein | + | 02399 | 5.5 | 6.6 |
| Hypothetical protein | + | 07067 | 5.1 | 6.4 |
| Arginine transporter ATP-binding subunit (artP) | - | 00689 | 3.1 | 6.2 |
| Hypothetical protein | + | 02676 | 4.1 | 6.1 |
| Hypothetical protein | + | 02408 | 3.7 | 5.7 |
| Metal dependent phosphohydrolase | + | 03800 | 4.2 | 5.5 |
| Hypothetical protein | + | 02424 | 3.5 | 5.4 |
| Hypothetical protein | + | 03676 | 4.0 | 5.2 |
| Putative sulfate transport protein (cysZ) | + | 35311 | 4.2 | 5.2 |
| Hypothetical protein | + | 02396 | 4.2 | 5.0 |
| Hypothetical protein | + | 02326 | 6.9 | 5.0 |
| Hemolysin A | - | 00011 | 7.7 | 4.9 |
| Mu-like phage gp25 | + | 02352 | 4.7 | 4.9 |
| Hypothetical protein | + | 02380 | 3.7 | 4.9 |
| Mu-like phage gp26 | + | 02365 | 5.3 | 4.8 |
| Mu-like phage gp27 | + | 02281 | 4.8 | 4.8 |
*The genes listed in Table 3 met both criteria that follow: a. The transcriptional level in human serum ≥ the median value among 2127 expressed genes based on RNA sequencing. b. The transcriptional level in human serum versus TSBYE ≥ 1.50.
The genes in bold belong to a four-gene operon artPIQM, a putative binding-protein transport system specific for arginine-uptake.
**Accessory genes (15 of 20): 15 genes are not present in two low-responder strains, D7S-1 and SCC1398.
***Detailed information of P-cluster is available in genomic database of A. actinomycetemcomitans: http://expression.washington.edu/genetable/script/gene_table_viewer.