Table 5. Protein composition comparison of the ~25 kDa bands (Fig 9) from the high-responder strain D11S-1.
| Proteins | P-cluster | Coverage (%)* | ||
|---|---|---|---|---|
| Human serum | Horse serum** | TSBYE | ||
| 3-ketoacyl-(acyl-carrier-protein) reductase [D11S-1] | 00698 | 90.1 | 90.5 | 92.2 |
| Phosphate transport regulator [D11S-1] | 01354 | 89.8 | 97.4 | 70.8 |
| Apolipoprotein A1 [HUMAN] | N/A | 81.7 | N/A | N/A |
| Hypothetical protein D11S_2268 [Phage S1249] | 15463 | 77.8 | 85.0 | 81.2 |
| Putative solute/DNA competence effector [D11S-1] | 00791 | 77.7 | 91.1 | 75.7 |
| Partition protein [Plasmid S57] | 02649 | 75.1 | 87.8 | 84.0 |
| Triosephosphate isomerase TpiA [D11S-1] | 00625 | 69.0 | 69.0 | 45.9 |
| Putative sulfate transport protein CysZ [D11S-1]*** | 35311 | 67.2 | 67.6 | Not detected |
| Hypothetical protein D11S_2271 [Phage S1249] | 03578 | 65.6 | 78.4 | Not detected |
| Complement component C8 gamma chain [HUMAN] | N/A | 64.4 | N/A | N/A |
| Hypothetical protein D11S_2218 [Phage S1249] | 15413 | Not detected | 98.1 | Not detected |
| Uracil phosphoribosyltransferase [D11S-1] | 00789 | 56.7 | 90.9 | 71.6 |
| Hypothetical protein D11S_2201 [D11S-1] *** | 03676 | Not detected | 90.3 | 52.2 |
| Cobyrinic acid a,c-diamide synthase [Plasmid S25] | 02657 | 62.4 | 90.2 | 71.2 |
| CmgB8 [D11S-1] *** | 03526 | 54.0 | 80.4 | 49.4 |
| Decarboxylase family protein Rossmann fold nucleotide-binding protein [D11S-1] | 00851 | Not detected | 59.8 | 84.7 |
| Acetyl-CoA carboxylase, biotin carboxyl carrier protein [D11S-1] | 05408 | Not detected | 78.1 | 82.6 |
| Ribonuclease T [D11S-1] | 01369 | Not detected | 71.4 | 71.9 |
*The proteins are listed according to the coverage of the corresponding protein sequences detected by LC/MS analyses. The 10 proteins with the highest coverage in each of the three samples: D11S-1/Human serum (coverage: 90.1%-64.4%), D11S-1/Horse serum (Coverage: 98.1%-80.4%) and D11S-1/TSBYE (Coverage: 92.2%-70.8%), corresponding to bands labeled with square brackets ‘[‘ in Fig 9.
**The data generated from LC/MS did not match the horse serum database, and proteins from horse serum may be present.
***Accessory genes (3 of 18): cysZ (P-cluster35311) also showed 4- to 5-fold up-regulated transcripts in horse or human serum versus TSBYE (see Table 3).
N/A: not applicable.