Fig 2. Brca2^G25R/Ko decreased testis size due to persistent DSBs and decreased localization of RAD51 in meiocytes.

A. Representative images of pachytene meiocytes of (B2^Ko/+, left, B2^GR/GR, center, and B2^GR/Ko, right) stained with SYCP3 (top, red), γH2AX (middle, green), and merged (bottom). B. Quantification of cells containing persistent γH2AX staining in pachynema. C. Representative images of zygotene meiocytes of (B2^GR/+, left, B2^GR/GR, center, and B2^GR/Ko, right) stained with SYCP3 (top, red), RAD51 (middle, green), and merged (bottom). D. Quantification of RAD51 foci along SYCP3 stained meiotic cores at zygonema. Error bars: SD, * p<0.05. Controls are represented by either: Brca2^+/+, Brca2^Ko/+, or Brca2^G25R/+. Abbreviations: Brca2^Ko/+ = B2^Ko/+, Brca2^G25R/+ = B2^GR/+, Brca2^G25R/G25R = B2^GR/GR, Brca2^G25R/Ko = B2^GR/Ko.