Fig 6. Brca2^G25R contributes to genomic instability and affects RAD51 foci formation.

A. Spontaneous chromosomal aberrations seen in MEFs of various genotypes (left) and total chromosomal aberrations seen in untreated and 100nM MMC treated MEFs (right). B. Chromosomal aberrations seen with in MEFS of various genotypes treated with 100nM MMC (left) and fold increase in aberrations seen after 100nM MMC treatment (right). Error Bars: SD, * p<0.05 C. Representative images of RAD51 and γH2Ax foci formation after irradiation in primary MEFs of various genotypes. D. Quantification of RAD51 foci seen in cells that also have γH2AX foci. Controls are represented by: Brca2^+/+ (with or without Palb2^Ko/+). Abbreviations: Brca2^+/+ = WT, Brca2^Ko/+ = B2^Ko/+, Brca2^G25R/G25R = B2^GR/GR, Brca2^G25R/Ko = B2^GR/Ko, Palb2^Ko/+ = P2^Ko/+.