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. 2016 Aug 4;11(8):e0160625. doi: 10.1371/journal.pone.0160625

Fig 6. Fluorescence imaging and flow cytometric analysis of live cells during neutrophilic differentiation of HL-60 cells.

Fig 6

a) HL-60 cells were differentiated into neutrophil-like cells by ATRA for 6 days. The cells were treated with 50 μM TAP-4PH for 30 min. Left panels, bright field; middle panels, TAP-4PH; right panels, merged images of bright field and TAP-4PH (cyan). Scale bar: 10 μm. b) Density plot showing TAP-4PH fluorescence and forward scatter in HL-60 cells before (left) and after (right) ATRA-induced differentiation. Black: undifferentiated HL-60 cells without TAP-4PH; blue: differentiated HL-60 cells without TAP-4PH; red: undifferentiated HL-60 cells treated with 50 μM TAP-4PH; green: differentiated HL-60 cells treated with 50 μM TAP-4PH.