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. 2016 Aug 4;11(8):e0160544. doi: 10.1371/journal.pone.0160544

Fig 4. SDS-PAGE and Western blot of bacteria from artificially contaminated cheese.

Fig 4

(A) SDS-PAGE (12%-acrylamide) showing the protein extracts from the bacteria after enrichment step using stomacher bags. Protein extracts from pure cultures were used as control. (B) Western blot using both mAb-3F8 and -2D12 on these protein extracts shows the expected detection bands: both InlA (88 kDa) and FBA (30 kDa) for the pathogenic strain; and only FBA (30 kDa) for the non-pathogenic. Purified rInlA and rFBA, and the pure cultures of L. monocytogenes and L. innocua were used as positive control for the antibodies.