Figure 4.
Expression Analysis of RCBTB1 mRNA
(A) Expression analysis was performed according to the manufacturer’s instructions with an in-house-designed custom array (SurePrint G3 Human Gene Expression array version 2, AMADID 041648, Agilent Technologies) covering all protein-coding genes and 22,980 long non-coding RNA transcripts (LNCipedia version 2.1). Data normalization was performed with the VSN package in R. All values were log2 transformed. Samples included total RNA from whole brain, colon, heart, kidney, liver, lung, breast, and adrenal gland (Stratagene Europe; all adult tissues); cerebellum, brain stem, striatum, frontal cortex, occipital cortex, and parietal cortex (Agilent; adult tissues); and fetal whole brain (Agilent).
(B) qPCR-based expression analysis of mRNA from RCBTB1 and two positive control genes strongly expressed in the retina and retinal pigment epithelium (RPE) was performed as previously described25 on commercial human cDNA from retina (BioChain) and RPE (3H Biomedical). High retinal and limited RPE expression was observed. Error bars represent the SE of the relative quantities.