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. 2016 Aug 2;99(2):489–500. doi: 10.1016/j.ajhg.2016.06.022

Figure 4.

Figure 4

Amount and Localization of DNAJB13 and RSPH23 in AECs and Spermatozoa from a Healthy Control Individual and Individual DCP813

(A) DNAJB13 (i and iii) was present in the cilia of AECs from a healthy control but absent from the cilia of individual DCP813. RSPH23 (ii and iv) was detected within cilia of AECs from both the healthy control individual and individual DCP813. Scale bars represent 5 μm.

(B) Western blot analysis shows the absence of DNAJB13 in AECs from individual DCP813 (control cells are shown for comparison). DNAI1, a component of the ODAs, was used as a positive control and was detected in cells from both the healthy control individual and individual DCP813.

(C) In contrast to control sperm (i), the sperm flagellum from individual DCP813 (iii) did not display DNAJB13, whereas RSPH23 co-localized with tubulin in the sperm flagellum from both the control individual and individual DCP813 (ii and iv). Scale bars represent 5 μm.