Proposed model for the negative feedback of vasopressin stimulated UT-A1 activity by 14-3-3ɣ. This schematic shows vasopressin (AVP) binding to the type 2 vasopressin receptor (V2R) and stimulating cAMP production, which stimulates PKA to activate/phosphorylate UT-A1. It also shows the PKA stimulation of 14-3-3ɣ which then binds to UT-A1 promoting ubiquitination (Ub) and subsequent degradation, effectively reversing the original PKA-mediated activation. The data showing these opposite effects of PKA are established, but whether there is a negative feedback loop, or whether only one effect is dominant in vivo, remains to be determined.