Regulation of NGF, BDNF, and VEGF transcript levels in littermate wildtype (WT) and in mice with chronic urothelial NGF overexpression (NGF-OE) in urothelium (A1–A3) and detrusor smooth muscle (B1–B3) with or without cyclophosphamide (CYP) treatment of varying duration (4 hours (h), 48 h, chronic). Relative expression of the urothelium (A1–A3) and detrusor (B1–B3) receptor transcripts are expressed as a percentage of WT urothelium and normalized to the relative expression of the housekeeping gene, 18S. A1, B1: NGF mRNA expression. A2, B2: BDNF mRNA expression. A3, B3: VEGF mRNA expression. Samples size are n of 6–8; *, p ≤ 0.05 versus control; #, p ≤ 0.05 between WT and NGF-OE.