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Mesenchymal stem cells (MSCs) were confirmed by scanning electron microscopy before transplantation. Cultured MSCs were seeded onto (A) a PLGA film (IP group), and (B) a chitosan fiber (IC group). The impregnated MSCs on the scaffolds were confirmed by immunofluorescence (IF) staining, which were shown to be PKH 26-positive (× 200).
