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Experimental protocol of our study.
Female C57BL/6 mice were treated with intratracheal instillation of bleomycin (BLM) or saline on day 0. Metformin (50 mg/kg or 100 mg/kg, according to group assignment) or PBS was administered orally once a day from day 1 onward. Half the mice were sacrificed on day 10 and the remaining mice were sacrificed on day 21.
