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. 2016 Aug 5;7:1240. doi: 10.3389/fmicb.2016.01240

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Copyright © 2016 Topçuoğlu, Stewart, Morrison, Butterfield, Huber and Holden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Average total CH₄ production in 2013 microcosms. The microcosms were incubated at 80°C (A) and 55°C (B) and amended with 200 kPa of H₂:CO₂ (red); 200 kPa of H₂:CO₂ plus 47 μM NH₄Cl (blue); and 2 kPa of H₂ and 198 kPa of N₂:CO₂ (black). The gray columns show the total CH₄ production for the four pure cultures in modified DSM 282 medium for comparison. The sample bars represent the range of the duplicate incubations.