Fig. 5.

Protein array methods for proteomic profiling. A, Direct labeling method adds detectable markers to the proteins, and uses antibodies fixed on a solid surface to capture the proteins of interest. B, Sandwich method utilizes two types of antibodies to capture the proteins and to tag on the fluorescent molecules respectively. C. Reverse phase protein array method first prints protein lysate on a solid surface, and then uses antibodies to quantify the proteins of interest.