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. 2016 May 23;15(8):2616–2627. doi: 10.1074/mcp.M115.056887

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 1. — Proteome analysis of oocytes from two different culture systems. (A) Conventional IVF culture system with oocyte culturing in a closed system–conventional IVF medium in covered four-well dishes (0.5 ml of medium per well). (B) New hanging drop culture system culturing oocytes in small, 8 μl droplets of DMEM/F12 medium. After culturing, both oocytes and droplets of corresponding medium were sampled to analyze the proteome and secretome. (C) Estimated abundance by iBAQ value of proteins identified in oocytes grown in IVF media and in hanging drops. Blue symbols: proteins identified both in IVF and hanging drops. Red symbols: proteins identified in hanging drops only.