FIGURE 3.

Expression of SCIMP and its regulation in dendritic cells and macrophages. A, BMMFs were differentiated from bone marrow cells for 7 days in M-CSF-containing medium. On day 7, the medium was replaced with GM-CSF-containing medium, and after an additional 24 h, cells were lysed in SDS-PAGE sample buffer. SCIMP was detected by immunoblotting. WB, Western blotting. B, dendritic cells were generated using Flt3L- and stem cell factor-containing medium. On day 8, plasmacytoid dendritic cells were removed using anti-mPDCA-1 magnetic beads, and the rest of the dendritic cells were cultured for 24 h in GM-CSF-containing medium followed by analysis of SCIMP expression by immunoblotting. C, splenic cDCs (CD11c⁺, B220⁻, Ly6C⁻) were sorted from the spleens of wild-type mice, lysed, and subjected to immunoblotting with SCIMP and GAPDH antibodies. BMDCs served as a positive control.